Frequently Asked Questions
Nanovesicles are isolated and concentrated through serial rounds of centrifugation and ultracentrifugation in a sterile condition.
PDNVs are characterized by Nanoparticle Tracking Analysis (NTA) to analyze number and size, and the expression of TET8 (Tetraspanin-8).
PDNVs are stable and functional in cell culture assays for about a week. This may vary under specific experimental conditions.
All isolated ITbio-PDNVs™ fall within the size range typical of plant-derived extracellular vesicles. Mean size of vesicles varies accordingly with the fruits or vegetables under study.
Each vial contains 2×1010 ITbio-PDNVs™.
ITbio-PDNVs™ are characterized for TET8 expression.
The number of nanovesicles are measured through Nanoparticles Tracking Analysis (NTA – NS300 Malvern Panalytical).
ITbio-PDNVs™ Ready to use in liquid form are suspended in 1 ml of sterile H2O. ITbio-PDNVs™ are shipped at +2-8 °C and can be stored at +4°C for a short time. It is recommended to aliquot PDNVs in the desired volume and store asap at either -20°C (active for up to 6 months) or -80°C (active for up to 1 year).
How are ITbio-PDNVs™ Ready to use in lyophilized form packaged and what is recommended for long term storage?
It is recommended to reconstitute PDNVs in sterile H2O or PBS and aliquot in different vials accordingly with the costumers’ best use. Freeze at -20°C/-80°C for long term storage. Avoid repeated freeze and thaw cycles.